Master’s Student Research in Biomedical Engineering on the topic of THE EFFECTIVENESS OF PHOTODYNAMIC THERAPY AND OZONE
FOR THE HEALING OF WOUNDS INFECTED WITH Methicillin Resistant Staphylococcus Aureus IN MICE. This research was conducted by Wahyu Intan Pertiwi, Str. Kep. The research abstract is as follows.
A wound is a disruption in the continuity of tissue at the cellular and anatomical levels, which can occur on the skin or mucosa and responds to the wound healing process. In this study, 112 subjects were randomized and divided into two groups: a control group and a treatment group. The treatment group received 5 types of therapy and the control group received 2 types of therapy, where the therapy group underwent red laser therapy, blue laser therapy for 60 seconds and ozone therapy for 30 seconds, as well as a combination of red laser & ozone therapy, and a combination of blue laser & ozone therapy with an irradiation dose of 3.5 J/cm2. The results showed that the wound healing process that received red laser therapy, blue laser therapy for 60 seconds and ozone therapy, as well as combined red laser and ozone therapy and combined blue laser and ozone therapy, obtained good results. Almost all of them had significant differences compared to the control group, which means that the control group that used red laser, blue laser, ozone, and their combination therapies showed effectiveness in wound healing as assessed by histopathology tests, lymphocyte and monocyte tests, bacterial colony counts, and wound length measurements. Meanwhile, the most effective results in wound healing using blue laser therapy combined with ozone were the most effective therapy in healing infected incision wounds, as evidenced by the One-way ANOVA test followed by the Post Hoc test, which showed significant results with p=0.05. Based on the average graph results, the combination of blue laser and ozone therapy was the most effective compared to other treatments, as seen from the histopathology test, lymphocyte test, monocyte test, bacterial colony test, and wound length measurement variables.
